This is necessary to avoid cross contamination of additives between the tubes. Green heparin lithium or sodium.
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Clsi order of draw is as follows.
Order of blood draw. Order of draw table. Gold sst mint green. By using the following mnemonic you ll have an easier time remembering the proper order.
So in order for blood to clot in safer plastic tubes manufacturers coat the inside of the tube with a substance to facilitate clotting like silica particles. As you may already know blood must be drawn and collected in tubes in a specific order known as the order of draw. Necessitating the change was the industry wide transition from glass blood collection tubes to plastic.
Sodium citrate tube eg blue closure serum tubes including those with clot activator and gels eg red red speckled gold closures heparin tube with or without gel eg dark green light green speckled green closures. Gold red gray sst gel w clot activator. Serum separator tube sst does not have any additive but a clot activator that will separate blood from the serum by centrifugation.
Gold sst plain tube w gel and clot activator additive green and dark green heparin with and without gel lavender edta pink blood bank edta gray oxalate fluoride list updated 02 11 2019. Whereas glass is a natural clot activator plastic is not. Luckily we ve composed a simple chart mnemonic and infographic to easily help phlebotomists remember the proper order.
Sodium polyanethol sulfanate aka sps sodium citrate. This draw is used in chemistry immunology and serology. Heparin either sodium or lithium e thylene d iamine t etra a cetic acid aka edta potassium oxalate with sodium fluoride.
Sterile blood culture tubes or vials. It is important to take the blood cultures first to prevent contamination from the additives of the other blood tubes. Considering the most common tubes used by nurses for venipuncture here is the proper order of blood draw.
Sterile technique is critical to obtaining a perfect specimen. Phlebotomy order of draw. The correct order of draw follows.
Unfortunately this order can be hard to remember for those in training and even those in the field. The specimens you should draw first are blood culture bottles and sps yellow top tubes. Due to the many colored top blood collection tubes it can often be difficult to remember the correct order.
1 blood cultures yellow sps sterile. Sterile specimens are drawn first to prevent contamination that can occur during the procedure. This order of draw has changed over the years the last occurring in 2003.
Order of draw when using evacuated tubes when collecting diagnostic blood specimens by venipuncture into evacuated tubes the order of draw is b ased on clinical and laboratory standards institute s clsi standard h3 a6 from november 2007 as follows. The draw order for specimen tubes is as follows. Blue tube for coagulation sodium citrate red no gel.
Blood culture tube or bottle.
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